The transferrin receptor is an integral membrane glycoprotein having a molecular weight of 190 kilodaltons kd. Mechanism of production of the serum transferrin receptor. Plasma ferritin and soluble transferrin receptor concentrations. Soluble transferrin receptor conventionally refers to the cleaved extracellular portion of transferrin receptor 1 that is released into serum. Cells can be treated with the ligand transferrin labeled with. The transferrin receptor is comprised of two identical subunits, each composed of 760 aminoacids with a molecular mass of 95 kdaltons. Cells can be treated with the ligand transferrin labeled with alexa fluor 546. The number of stfr reflects the cellular requirements for iron, and varies with the function and the morphological development of each cell type. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia. Online conversion calculator for many types of measurement units in laboratory and medicine soluble. Summary of test principle and clinical relevance the method principle for measurement of soluble transferrin receptor stfr is immunoturbidimetry using roche kits on the hitachi 912 clinical analyzer. Purchase human soluble transferrin receptor,stfr elisa kit.
The soluble transferrin receptor stfr test is primarily ordered to help distinguish between anemia that is caused by iron deficiency and anemia that is caused by inflammation or a chronic illness socalled anemia of chronic disease or inflammation. In contrast, soluble transferrin receptor stfr concentrations generally begin. Feb 12, 2014 transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. Transferrin receptor tfr is a carrier protein for transferrin. Evaluation of suspected iron deficiency in patients who may have inflammation, infection, or chronic disease and other conditions in which ferritin. It imports iron by internalizing the transferrin iron complex through receptor mediated endocytosis. Pdf the usefulness of soluble transferrin receptor stfr.
Role of soluble transferrin receptor and transferrin receptor. Soluble transferrin receptor in serum nhanes 20032004 page 3 of 12 1. Thus, an efficient sample pretreatment strategy is required. Transferrin receptor tfr the transferrin receptor tfr is the gateway for transferrinboundiron entering all body cells. Soluble serum transferrin receptor can be a helpful test in the diagnosis of ida in patients with inflammatory. Development of a human soluble transferrin receptor elisa. Transferrin receptor gene and protein expression and localization in human iugr and normal term placentas. Scott1 soluble transferrin receptor stfr and ferritin concentrations were measured in a variety of clinical settings to. May 05, 2006 production of the soluble portion of the transferrin receptor stfr by baby hamster kidney bhk cells is described, and the effect of glycosylation on the biological function of stfr is evaluated for the first time. Soluble transferrin receptors and iron deficiency, a step. Levels of this receptor are increased in irondeficiency anemia. Soluble transferrin receptor is elevated in association with hyperplastic erythropoiesis, for example, in patients with haemolytic anaemia or betathalassaemia.
Case 1 a 46yearold female with a history of rheumatoid arthritis presents to you with complaints of heavy menses and fatigue. Transferrin is a protein present in blood that binds to iron. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of irondeficiency anemia. Summary of test principle and clinical relevance the method principle for measurement of soluble transferrin receptor stfr is. After adding the product to the cart by clicking the button below you will see if there is a volume discount available. Tfr is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body tfr content. Soluble transferrin receptor article about soluble. The serum concentration of stfr reflects the amount of the cellular receptor 1,2.
In patients with acd alone, the ratio is 2 table 2. A proteolytic product of the transferrin receptor circulates in plasma as soluble transferrin receptor stfr. Serum transferrin receptor levels for the assessment of iron status. Transferrin receptor an overview sciencedirect topics. However, stfr levels in some situations could be far below the limit of quantification loq of most assays.
Soluble transferrin receptors and iron deficiency 347 the endosomes acidosomes, and the tftfr complex is returned back to the cell surface, where apotransferrin is released again 41. Soluble transferrin receptors stfr are proteins found in blood that can become elevated in the presence of irondeficiency. Functional iron deficiency fid exists when, despite adequate stores, iron cannot be mobilised for erythropoiesis, mediated by elevated hepcidin. Order name solubl trn test number 3604710 revision date 03032009. Soluble transferrin receptor discriminates iron deficiency anemia from anemia of chronic disease in leukemic and breast cancer patients.
On monday, december 17, 2001, the clinical chemistry laboratory of the department of pathology will begin accepting specimens for the soluble transferrin receptor assay stfr. Soluble transferrin receptors stfr were detected in culture supernatants of activated human peripheral blood mononuclear cells pbmc using a sandwich elisa technique with two noncrossreacting tfr moabs. It is needed for the import of iron into the cell and is regulated in response to intracellular iron concentration. Soluble transferrin receptor stfr in serum has been suggested as a marker for breast cancer diagnosis, monitoring and treatment. Therefore, serum transferrin receptor levels reflect the intensity of red blood cell formation. Transferrin receptor is a nonrtk constitutively recycling receptor and thus can be used as a control to assess rtk specificity of met trafficking. Soluble transferrin receptor stfr conversion to nmoll. The method principle for measurement of soluble transferrin receptor stfr is immuno. Soluble transferrin receptor stfr and stfrlog ferritin index for.
Tfr is abundant on the surface of many newly formed cells, but the erythroid marrow. Please refer to the roche cobas 6000 operators manual and the sop. Soluble transferrin receptor an overview sciencedirect. Mitogenic stimulation of lymphoid cells induced both upregulation of tfr surface density and release of stfr to the medium. Soluble transferrin receptor centers for disease control cdc. The prevalence of iron depletion, iron deficient erythropoiesis ide, and iron deficiency anemia ida was assessed in preschool inuit children using soluble transferrin receptor stfr and traditional. In the past 18 months, her periods have become increasingly heavy for 35. It consists of two identical subunits linked by disulfide bridges. William f hancock, md 1447 york court, burlington, nc 27215. Pdf measurement of soluble transferrin receptor in serum of.
Recent studies have also suggested that stfr measurements are helpful when predicting the response to erythropoietin in patients with anaemia resultant from chronic renal failure. Pdf the concentration of soluble transferrin receptor stfr in serum is reported to be useful in the diagnosis of iron deficiency, especially for. An additional iron index is the soluble transferrin receptor stfr concentration, which is elevated in tissue iron deficiency. As transferrin expression increases with decrease in iron concentrations,measurementofitssoluble form can be a valuable indicator of iron deficiency. Soluble transferrin receptor regional medical lab test. Soluble transferrin receptor levels may be helpful in distinguishing iron deficiency from anemia of chronic inflammation in cf because, unlike serum ferritin and transferrin, soluble transferrin receptor does not. Pdf we evaluated the usefulness of soluble transferrin receptor stfr and of the stfrlog ferritin indexstfrlogf in the differentiation of anemia.
The reference range of transferrin receptor varies by. Transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. Transferrin receptor testing is used to measure the level of soluble transferrin receptors. Soluble transferrin receptor and ferritin concentrations were nephelometrically determined by n latex reagent sets bade behring according to the manufacturers instructions after storage of the. Determination of serum soluble transferrin receptor stfr is proposed to distinguish between irondeficiency anemia and. Soluble tfr is a truncated monomer of the tissue receptor, lacking its first 100 amino acids, which circulates in the form of a complex of transferrin and its receptor. Effect of glycosylation on the function of a soluble.
This receptor is a protein dimer of two identical subunits, linked. This test measures the amount of soluble transferrin receptors within the blood, to. Soluble transferrin receptor is an important monitoring tool to trend id in the intestinal failure population patients who receive a high percentage of their calories via parenteral nutrition pn are more susceptible to iron deficiency, especially infants and children. After adding the product to the cart by clicking the button below you will see if there. Pdf analytical and clinical implications of soluble. Iron is an essential nutrient absorbed from the diet. In contrast, soluble transferrin receptor stfr is elevated in iron deficiency but is not appreciably affected by chronic disease.
Soluble transferrin receptor levels may be helpful in distinguishing iron deficiency from anemia of chronic inflammation in cf because, unlike serum ferritin and transferrin, soluble transferrin receptor does not appear to be an acutephase reactant. Soluble transferrin receptor an overview sciencedirect topics. Soluble transferrin receptor and transferrin are measured in plasma and serum by immunoassays based on the specific antitf or antitfr antibodies. Soluble transferrin receptor definition of soluble. Soluble transferrin receptor, human, in vitro, antibody. Each of the monomers has an 85 kd cterminal component which can bind an ironladen transferrin molecule. The higher the body iron stores, the lower the transferrin receptors in cell membranes which in turn downregulates of intestinal iron absorption 9. Soluble transferrin receptor tests result flag units reference interval lab soluble transferrin receptor 30. Soluble transferrin receptorferritin index in the evaluation. Production of the soluble portion of the transferrin receptor stfr by baby hamster kidney bhk cells is described, and the effect of glycosylation on the biological function of stfr is. Soluble transferrin receptor stfr is a truncated form of the cellular transferrin receptor, without its transmembrane and cytoplasmic domains, and circulates bound to transferrin. This test measures the amount of soluble transferrin receptors within the blood, to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anaemia. The stfr reflects the iron demand of the body and is postulated as a regulator of iron homeostasis via binding to the hereditary hemochromatosis protein hfe. Soluble transferrin receptors and iron deficiency 347 the endosomes acidosomes, and the tftfr complex is returned back to the cell surface, where apo transferrin is released again 41.
Clinical utility of the soluble transferrin receptor and. Levels of this receptor are increased in irondeficiency. However, stfr levels in some situations could be far. Using soluble transferrin receptor and taking inflammation. This receptor is a protein dimer of two identical subunits, linked together by two pairs of disulfide bonds. When the number of complexes decrease, the number of vacant stfrs increase. Transferrin receptor is a transmembrane glycoprotein expressed by any cell type except erythrocytes. Soluble transferrin receptor and ferritin concentrations were nephelometrically determined by n latex reagent sets bade behring according to the manufacturers instructions after storage of the centrifuged 2600g.
A standard sandwich assay sa for the detection of soluble transferrin. Clinical utility of the soluble transferrin receptor and comparison with serum ferritin in several populations alan e. The detection of a soluble form of the transferrin receptor tfr in human sera has been a major advance in hematological and ferrobiological research. Soluble transferrin receptor stfr conversion to nmoll, mg. The transferrin receptor tfr is a homodimeric type ii membrane protein that mediates iron uptake into the cells. Thus, the concentration of stfr in plasma or serum is an indirect measure of total tfr. The soluble transferrin receptor stfr is the truncated form of the transmembrane receptor which. Soluble transferrin receptors are proteins found in blood that can be elevated with iron deficiency. This bound complex then binds to soluble transferrin receptors stfrs on cells. Laboratory procedure manual centers for disease control. If the inline pdf is not rendering correctly, you can download the pdf file here. Oct 23, 2015 soluble transferrin receptor stfr in serum has been suggested as a marker for breast cancer diagnosis, monitoring and treatment.
The significance of soluble transferrin receptors in diagnosing iron. When the number of complexes decrease, the number of. Measurement of circulating levels of the receptor are very useful in the diagnosis of iron deficiency in patients who also have anemia. Soluble transferrin receptor stfr is a circulating, cleaved form of a membrane receptor protein. In blood, soluble tfr is completely bound to tf and. A standard sandwich assay sa for the detection of soluble transferrin receptor stfr, a biomarker of ida, on a photonic crystal pc biosensor was established, but it was susceptible to nonspeci. In this crosssectional study, parameters for anthropometry, lipids and iron metabolism including transferrin, soluble transferrin receptor stfr, ferritin and serum iron sf were evaluated. Proteolysis leads to the soluble form of the transferrin. Hemoglobinhb, serum ferritin and soluble transferrin receptor stfr levelswere performed on children aged 6 59 months. Elevated stfr values are found in anemia of chronic disease acd, iron deficiency anemia ida, polycythemia. Soluble transferrin receptor stfr useful for evaluation of suspected iron deficiency in patients who may have inflammation, infection, or chronic disease and other conditions in which ferritin concentration does not correlate with iron status, including. In recent years, soluble transferrin receptor stfr has been introduced as a sensitive, early and highly quantitative new marker of iron depletion, increasing in proportion to tissue iron deficit. Soluble transferrin receptor arises from proteolysis of the intact protein on the cell surface, leading to monomers that can be measured in plasma and serum.
The human transferrin receptor tfr is shed by an integral metalloprotease releasing a soluble form stfr into serum. The stfr reflects the iron demand of the body and is. The concentration of soluble transferrin receptor stfr has been reported to be proportional to the total amount of cellassociated transferrin receptor. As a result, these stfrs are released into the blood stream. Soluble transferrin receptor stfr test test results, normal. Online conversion calculator for many types of measurement units in laboratory and medicine soluble transferrin receptor stfr, stfr, transferrin receptor, tfr, serum transferrin receptors. Soluble transferrin receptor stfr test test results.
As transferrin expression increases with decrease in iron concentrations,measurementofitssoluble form can be a valuable indicator of iron. Pdf soluble transferrin receptor discriminates iron. Feb 28, 2011 soluble transferrin receptor stfr is a truncated form of the cellular transferrin receptor, without its transmembrane and cytoplasmic domains, and circulates bound to transferrin. Soluble transferrin receptor is an important monitoring tool to trend id in the intestinal failure population patients who receive a high percentage of their calories via parenteral nutrition pn are more. Human soluble transferrin receptor,stfr elisa kit cusabio. The soluble transferrin receptor stfr test is primarily ordered to help distinguish between anemia that is caused by iron deficiency and anemia that is caused by inflammation or a chronic illness socalled. A soluble form of the human transferrin receptor is. Soluble transferrin receptor, human, in vitro, antibody cat. In patients with acd alone, the ratio is tfr the transferrin receptor tfr is the gateway for transferrinboundiron entering all body cells. Efficient molecular imprinting strategy for quantitative. The amount of transferrin receptor expressed on a cell is proportional to the cells need for iron. Soluble transferrin receptor stfr, serum barnesjewish. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency.
Diagnosis and management of iron deficiency anaemia. Is soluble transferrin receptor a good marker of iron. Release of the soluble transferrin receptor is directly. Use of multiple antibiotics in patients with cf is routine. Soluble transferrin receptor stfr conversion to nmoll, mgl, mgdl, mg100ml, mg%, gml. The uptake of iron by cells is mediated by a cellsurface transferrin receptor tfr. Iron metabolism and its association with dyslipidemia risk. Latex bound antistfr antibodies react with the antigen in. Pdf the usefulness of soluble transferrin receptor stfr in.
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